180 THE EVOLUTION OF LIFE 
car ils peuvent encore renfermer des germes vivants 
qui se développent dans les liquides neutres.” 
I have given my interpretation of Pasteur’s failure 
in the repetition of my experiment, and itis of sucha 
kind as fully to account for his invariably obtaining 
negative results. It remains, therefore, for me now to 
adduce the various kinds of evidence which strongly 
negative the interpretation he has given of the results 
obtained by me. ‘They are as follows :— 
1. Pasteur’s explanation of my positive results 
may be disproved by direct experiment in the 
following manner, as I pointed out in my paper in 
the Journal of the Linnean Society (p. 45). 
We may take some turbid urine having a neutral 
or faintly alkaline reaction, in which both Bacteria 
and Bacilli are swarming and rapidly multiplying, 
and we may compare the relative effects of heating 
these organisms, with their reproductive particles, to 
212° F. in a neutral and in an acid medium respec- 
tively. Three series of experiments are needed :— 
1. Herment-organisms heated to | 2. Ferment-organisms heated to 
212° F. 2 an Acid Fluid. | 212° F. 2x a faintly Alkaline Fluid. 
Add one minim of the faintly alka- Place one minim of the same faintly 
line turbid fluid containing ferment- alkaline turbid fluid in a little tube, 
organisms and their germs to one | draw out its neck and seal. 
ounce of urine whose acidity is more | Immerse this tube in a vessel con- 
than equivalent to IL x of liquor po- | taining one ounce of the same 
tassx. | specimen of urine. 
Boil 2’ over the flame, seal, and | Boil 2’, seal, boil 8’ in same man- 
then 8’ in boiling water with the | ner, and when the fluid is cool, break 
flask or retort in an inverted position. | the tube so as to liberate organisms 
When cool place in incubator at | which have been heated in their own 

a2 ¥. Saintly alkaline medium. 
Aesult.—No change in fluid. Place in incubator with others at 
22°F. 
Result,—No change in fluid. 
