
OTHER NEW EXPERIMENTS 181 
, 3. Control Experiment. 
Boil an ounce of the same acid urine in a small flask, whose neck is plugged 
with cotton-wool, for 10’. 
When fluid has cooled remove cotton-wool plug for an instant, and add one 
minim of the same turbid fluid, but not previously heated. Quickly replace 
cotton plug and transfer to incubator at 122° F, 
Result. —Well-marked turbidity and swarms of Bacilli in 18- -24 hours. 
Such experiments have invariably given the same 
results. Twelve trials were made with a urine of 
1030 sp. gr., whose acidity was equal to 10 minims 
of liquor potassze per ounce; and nine trials were 
made with a urine whose acidity equalled 25 minims 
per ounce, and whose sp. gr. was 1030. 
These experiments are of much interest, because 
they show in a most decisive manner that the mere 
neutrality or slight alkalinity of the medium in 
which the ferment-organisms are heated is quite 
unable to preserve them from the destructive 
influence of a temperature of 100° C. They show 
also that Bacilli, zo¢ previously bozled, develop and 
multiply with great freedom even in a very highly 
acid urine. 
2. But the proof is also abundant and absolute 
that Bacilli and their germs are capable of de- 
veloping and multiplying after they have been 
botled in acid fluids. I need only refer to my 
experiments with simple unaltered acid turnip 
infusion in proof of this—experiments which have 
been confirmed by Burdon Sanderson as well as by 
Professor Huizinga. Taken at their lowest value, 
such as my opponents favour, these experiments are 
a direct disproof of the explanation given by Pasteur, 
and later by Chamberland, of my urine and liquor 
