OTHER NEW EXPERIMENTS 185 

was boiled the longest became turbid the most 
rapidly. 
Again, I tried this experiment with another 
specimen of urine (requiring eleven minims of liquor 
potasse for the neutralisation of one ounce) which 
was collected with every precaution in a flamdbé 
receiver and passed into two flasks in which tap 
water had been boiled for to’. They were placed 
in an incubator at 112° F., when one of them 
became turbid in twenty-four and the other in thirty- 
six hours, and on examination each fluid was aga 
found to contain Micrococci only. 
Two other flasks were treated in the same way, 
and some of the same stock of urine was added 
after the 10’ boiling, but now super-heated liquor 
potasse sufficient for neutralisation was added to 
each flask before it was plugged and placed in the 
incubator. The result was that both became turbid 
in twenty hours, and in each flask there were found 
multitudes of Micrococci, together with Streptococci 
and Bacilli of all sizes up to long Leptothrix-like 
threads. 
In three more experiments with a different urine, 
in which tap water was boiled in the flasks for ten 
minutes before the pure urine was added (without 
neutralisation), Micrococci only, again appeared in 
21 to 36 hours. 
From Pasteur’s point of view these results were 
very remarkable. The fluids, in order to accord 
with his interpretation of my experiments, ought in 
each case to have yielded Bacztd only, and neither he 
nor any one else has ever attempted to show that 
