OTHER NEW EXPERIMENTS 187 

end of the fourth day the fluids in the eleven flasks 
were still quite clear. Using all precautions, each 
flask was then inoculated with a single drop of urine 
turbid with Bacilli, with the result that in seven of 
the eleven flasks, the fluids became turbid with 
Bacilli in from 20 to 36 hours. The fluids in the 
other four flasks remained clear for another three 
days, and were not kept longer under observation. 
(c) Can pure Urine in flambé vessels, and guarded 
Jrom contamination, be made to ferment ? 
In, dus: Note of July. 17,1876, M.. Pasteur 
attached little importance to the high incubating 
temperatures which I have employed; moreover, he 
said that potash in proper proportions may be added 
even to unboiled urine, when passed with all proper 
precautions into a super-heated vessel, without causing 
it‘to ferment. And in this Note of January 8, 1877, 
written in collaboration with M. Joubert, he said the 
discussion concerns a fact, viz., ““whether urine which 
has been boiled so as to be sterile, and better still 
fresh urine, unaltered, as it passes from the bladder, 
will after neutralisation by potash, and at a tempera- 
ture of 50 C., produce organisms.” Pasteur con- 
tended that no fermentation would occur, so long 
as the potash added was pure. 
During the year 1883, when I took up the 
question of the power of resisting heat possessed by 
spores of Bacilli which had been in a state of 
desiccation for over five years, in reply to Professor 
Tyndall’s assumptions, I also performed a very 
