OTHER NEW EXPERIMENTS 189 

(d) The Effect of adding liquor potasse, in different 
proportions and with different Incubating Tem- 
peratures, to pure urine in flambé vessels. 
On very many occasions pure urine passed into a 
flambé vessel, if fully or two-thirds neutralised 
with superheated liquor potassa, and subsequently 
exposed to temperatures ranging from 116°-122° F. 
(44°-50° C.), was found to become turbid more rapidly, 
that is, in seventeen to twenty-four hours and, on 
examination, to show no Micrococci but swarms of 
Bacilli. 
On the other hand, if lesser amounts of liquor 
potassze were used, sufficient for half-neutralisation, 
or a little less or more, together with incubating 
temperatures between 117° and 116° F. (44°-47° C.) 
the fluids would generally yield a mixture of 
Micrococci, Streptococci, and Staphylococci, together 
with Bacilli varying much in size. 
I thus found that using pure urine in flamdé 
vessels, without or with liquor potasse in different 
proportions, and at different incubating tempera- 
tures, I could, almost at will, procure Micrococci 
alone, Bacilli alone, or a mixture of these with Strep- 
tococci more especially. 
In all the experiments where liquor potassz was 
used, I had—in order to eliminate all objections, 
however futile—previously heated it in tubes to 
105-120 C. for one hour, as I had formerly done 
to meet unfounded objections made by Pasteur in 
regard to the original experiments. 
