194 THE EVOLUTION OF LIFE 
In seven of these cases the fluids did not become 
generally turbid, though one or more tufts of Bacilli 
appeared in each: these were all specimens of the 
same urine which had been heated to 230° F. for 5 
minutes. In the other seven instances there was 
very slight general turbidity, and the vessels had 
been heated to 230° F. for 30 minutes. 
In twelve trials with faintly acid deer-wort, partly 
of 1060 specific gravity and partly diluted to 1030, 
heated in tubes with air to 230 F. for 10 minutes, 
fermentation did not once occur; though, as in the 
experiments with urine, the fluids were, after this 
superheating, kept for many days at a temperature 
olage: T 
The results with Zay-infusion, acid,’ neutral, and 
faintly alkaline, having a specific gravity of about 
1005, have been much more successful. In each 
case about half an ounce of the fluid was used, half- 
filling a tube which was sealed when cold; so that 
above the fluid there was ordinary air. After heating 
the tubes in the calcium bath, some of them were 
exposed in the incubator to temperatures between 
104-113. F. (40°- 45° C.), and others to a tempera- 
ture of 122° F, 
In fourteen cases the infusions were heated to 
230 F. for five minutes; and in every oneer 
these tubes organisms showed themselves within 
36 hours. In twenty-one cases they were heated to 
248° F. (120° C.) for 30 minutes; and in five of 
these latter trials (all with the same hay-infusion) 
1 Only three times in this state. In the other cases liquor potassz 
was added so as to make it neutral or slightly alkaline. 
