
EXPERIMENTS WITH SALINE SOLUTIONS 255 
from sealed tubes previously heated to 130° C. for 
twenty minutes. No such bodies are to be found 
depicted in any of the bacteriological works to 
which I have referred. The loculi are, of course, 
something like what is to be found in Leuconostoc, 
which Lehmann and Neumann say (4c. cz¢., p. 123) 
‘is only a Streptococcus, with, at times, enormously 
thick capsules.” One of its forms, too, is spoken of 
(p. 151) as assuming a “frog-spawn’”’-like mode of 
growth, which, on an extremely minute scale, is not 
unlike what is to be seen in Fig. 8. But then in our 
organism the contents of the loculi are Micrococci 
and not Streptococci. No such twisted threads are 
known to issue from Streptococci—nor, indeed, pre- 
viously from any other Cocci, so far as I have been 
able to ascertain. ‘The threads seem to be provided 
with no dissepiments; they are, therefore, not like 
the mycelia of a Mould, and seem to resemble much 
more closely the filaments of an Actinomyces. These 
are surely new organisms found in a new habitat— 
and it may well be asked, whence have they come? 
Results obtained with Saline Solutions which had 
previously been heated in hermetically Sealed 
Tubes to 100 C. for ten minutes. 
We now come to experiments of a stricter order, 
where previously sterilised tubes have been used, 
which, when charged with the experimental fluids, 
have been hermetically sealed, before boiling them 
in a can of water for ten minutes. 
In one of these tubes, charged with some of the 
