FINAL DECISIVE EXPERIMENTS 261 

the Pasteur Institute has declared, in regard to these 
spores in all such fluids, that “A temperature of 11 5 
C. sterilises them completely and most rapidly.” Our 
final trials must, therefore, be directed to ascertain- 
ing what results can be obtained with the different 
saline solutions, dealt with in the last chapter, when 
they have been exposed to temperatures ranging 
from 115-130 C. 
Results obtained with Saline Solutions whieh had 
previously been heated in hermetically Sealed 
Tubes to 115° C. (239 £.) for ten minutes. 
The experiments now to be referred to have all 
been made with A and AA solutions. In these 
experiments, as well as in others in which higher 
temperatures have been employed, the sealed tubes 
have invariably been placed in the calcium chloride 
or the oil bath, when this was cold, so that the 
tubes and their contents have been heated gradually 
with the bath, till the desired temperature has been 
attained. 
At the expiration of the ten minutes the tubes 
have been removed from the bath with a large 
wooden forceps, and allowed to cool. They have 
then been cleaned, labelled, and either placed in an 
incubator at 95° F., or exposed to diffuse daylight 
at a temperature which has sometimes been 35, 
or even 40 F., lower than this. Yet, at the 
expiration of five or six weeks, when the contents 
of the tubes were examined, living organisms have 
been found in those tubes exposed to the latter 
