
FINAL DECISIVE EXPERIMENTS 267 
media themselves, when they are slightly different 
in constitution or have been exposed to slightly 
different conditions. See also p. 274 and Pl. XII. 
in reference to this point. 
In another tube charged with the AA solution 
which, after superheating, had been kept in the 
incubator for six weeks, there was a similar paucity — 
of inorganic concretions distributed through the 
flakes. Bacteria were also very scarce, and only 
a few incipient Moulds were seen. One of these is 
represented in Fig. 25, B, while another much 
more developed specimen is shown in C, a portion 
of which (to the right of the centre) being invisible 
owing to its being below the proper focus. 
In two specimens of the B solution which had 
been heated to 125° C. for ten minutes, and subse- 
quently kept in the incubator for five weeks, no 
distinct organisms were found, though there were 
a number of bodies of uncertain nature. One of 
these is shown in Fig. 24, B. Another exactly like 
it was seen, together with many smaller bodies of 
like kind, of different sizes, without the bud-like 
outgrowth or projection. 
Results obtained with Saline Solutions whith had 
previously been heated in hermetically sealed 
Tubes to 130 C. (266° F.) for twenty minutes. 
Two tubes having been charged with the AA 
solution, were exposed to light for the first three 
months, and after that were placed in the 
incubator. The contents of one of them was 
