270 THE EVOLUTION OF LIFE 
the liquor ferri pernitratis to the ounce of distilled 
water (together with six drops of the sodium silicate 
solution). In the first experiment the tube was of 
soft German glass, while in the second a uviol tube 
was employed. 
The sediment in the first tube was of a pale yellow- 
ish colour, and the fluid above was of the same tint 
and slightly opalescent. When opened, after an 
exposure for twelve weeks to diffuse daylight, the 
fluid was found to be slightly acid. On examination 
of some of the sediment three small mycelia were 
found, with rather broad hyphz. No unmistakable 
Torule or Micrococci were seen, but there were a 
large number of Vibriones in many places, thickly 
scattered through the substance of the small granular 
flakes of which the sediment was largely composed. 
Some of these bodies, slightly stained with gentian 
violet, are shown in Plate X., Fig. 29, A, while in B 
unstained specimens are to be more dimly seen 
diffused through the substance of a granular flake. 
These bodies were almost exactly like those shown 
i Plate TV, Pigs, “12 and £3. 
The tube was closed after two specimens had 
been taken from it with a sterilised pipette, and was 
then left in a box for five weeks, when its contents 
were again examined. There seemed to be a 
distinct increase in the number of the Vibriones, but 
no other change was recognised, so the tube was not 
kept longer. 
After the uviol tube had been exposed to light for 
twelve weeks, it was placed in the incubator for 
another two weeks, and was then opened. The 
