
FINAL DECISIVE EXPERIMENTS 271 
fluid and sediment were similar to the last in appear- 
ance, but the reaction of the fluid was neutral. On 
microscopical examination of some of the sediment 
a single small incipient Mould was found, while 
Vibriones, as in the last tube, were fairly numerous 
in the substance of the granular flakes. No other 
organisms were found, and the tube was not kept 
longer for further observation. 
In the next two experiments to be referred to the 
ordinary BB solution was used. Both tubes were of 
the ordinary German glass. The fluid and sediment 
presented the same appearance as before. One of the 
tubes was opened after it had been exposed for twelve 
weeks to daylight during very hot weather (from June 
Ist to August 25th). The reaction of the fluid was 
faintly acid, and in the first two samples of the sedi- 
ment examined I found many aggregates of Vibriones 
such as are shown in Fig. 29, and also many groups 
of Micrococci, both free and loculated. From some 
of the groups of free Micrococci filaments, either 
straight or twisted, were seen growing; while the 
loculated Cocci, the early stages of which are shown 
in Fig. 30, A and B, were almost exactly like those 
represented in Plate III., Figs. 8, 9, and 10, froma 
similar solution that had been heated only to 1oo° C. 
in a corked flask. One very large sponge-like mass 
of such loculated Micrococci was also found embedded 
in, and somewhat obscured by, the substance of a 
granular flake. This is represented, under a lower 
power, in Fig. 30, C. In the lower right-hand 
border its constituent groups of Micrococci can be 
best made out. 
