270 THE BLOOD. 



Hcemoglobin from the C H N S Fe O PoOs 



Dog 53.85 7.32 16.17 0.390 0.430 21.84 (HOPPE-SEYLER) 



" 54.577.2216.380.5680.33620.93 (JAQUET) 



Horse 54.87 6.79 17.31 0.650 0.470 19.73 (KOSSEL) 



" :.. 51.156.7617.940.3900.33523.43 .... (ZINOFFSKY) 



Ox 54.66 7.25 17.70 0.447 0.400 19.543 .... (HUFNER) 



Pig 54.17 7.38 16.23 0.660 0.430 21.360 .... (OTTO) 



" 54.71 7.38 17.43 0.479 0.399 19.602 .... (HUFNER) 



Guinea-pig 54.12 7.36 16.78 0.580 0.480 20.680 .... (HOPPE-SEYLER) 



Squirrel 54.097.3916.090.4000.59021.440 



Goose 54.267.1016.210.5400.43020.6900.770 



Hen 52.477.1916.450.8570.33522.5000.197 (JAQUET) 



That the repeatedly observed quantity of phosphorus in the haemo- 

 globin of birds (Inoko and others) is due to a contamination has been 

 proven by ABDERHALDEN and MEDIGRECEANU. In the haemoglobin 

 from the horse (ZINOFFSKY), the pig, and the ox (HUFNER) we have 

 1 atom of iron to 2 atoms of sulphur, while in the haemoglobin from the 

 dog (JAQUET) the relation is 1 to 3. From the data of the elementary 

 analysis, as also from the amount of loosely combined oxygen, HUF- 

 NER 1 has calculated the molecular weight of dog-haemoglobin as 14,129, 

 and the formula CeseHK^Ni^FeSsOigi. According to the more recent 

 determinations of HUFNER and JAQUET, 2 ox-haemoglobin contains an 

 average of 0.336 per cent iron, from which a molecular weight of 16,669 

 may be calculated. HUFNER and GANSSER S have attempted to learn 

 the size of the molecular weight of haemoglobin by means of osmotic 

 pressure determinations, and they found the following approximate 

 results: for horse haemoglobin 15,115 and for ox-haemoglobin 16,321. 

 The haemoglobin from various kinds of blood not only shows a diverse 

 constitution, but also a different solubility and crystalline form, and a 

 varying quantity of water of crystallization; hence we infer that there 

 are several kinds of haemoglobin. BOHR is a very zealous advocate 

 of this supposition. He has been able to obtain haemoglobins from dog- 

 and horse-blood, by fractional crystallization, which had different powers 

 of combining with oxygen and contained different quantities of iron. 

 HOPPE-SEYLER had already prepared two different forms of haemoglobin 

 crystals from horse-blood, and BOHR concludes from all these observa- 

 tions that the ordinary haemoglobin consists of a mixture of different 

 haemoglobins. In opposition to this statement, HUFNER 4 has shown 



1 Hoppe-Seyler, Med. chem. Untersuch., 370; Jaquet, Zeitschr. f. physiol. Chem., 

 14, 296; Kossel, ibid., 2, 150; Zinoffsky, ibid., 10; Hufner, Beitr. z. Physiol., Festschr. 

 f. C. Ludwig, 1887, 74-81, Journ. f. prakt. Chem. (N. F.), 22; Otto, Zeitschr. f. physiol. 

 Chem., 7; Inoko, ibid., 18; Abderhalden and Medigreceanu, ibid., 59. 



2 Arch. f. (Anat. u.) Physiol., 1894. 



3 Arch. f. (Anat. u.) Physiol., 1907. 



4 Bohr, " Sur les combinaisons de 1'hemoglobine avec 1'oxygene," Extrait du 

 Bulletin de l'Acad6mie Royale Danoise des sciences, 1890; also Centralbl. f. Physiol., 

 1890, 249. Hoppe-Seyler, Zeitschr. f. physiol. Chem., 2; Hufner, Arch. f. (Anat. u.) 

 Physiol., 1894. 



