716 URINE. 



The acid which is found in large quantities, especially in the urine of 

 dogs poisoned with phosphorus (BONDZYNSKI and GOTTLIEB), is considered 

 like the preceding acid as an intermediary oxidation product of the pro- 

 teins, and oxyproteic acid seems to represent a higher state of oxidation 

 or a demolition of the proteins than the ant oxyproteic acid. 



The acid called uroproteic acid by CLOETTA is probably a mixture of several 

 bodies, according to the recent investigations of BONDZYNSKI, DOMBROWSKI, and 

 PANEK. The same applies also to the barium oxyproteate prepared by PREGL l 

 from the urine. 



Alloxyproteic acid is a thiid acid related to the above, which was first 

 isolated by BONDZYNSKI and PANEK 2 from the urine and then carefully 

 studied with DOMBROWSKI. The composition is: C 41.33, H 5.70, N 13.55, 

 S 2.19, and O 37.23 per cent, based upon new investigations. The free 

 acid is soluble in water. It gives neither the biuret reaction nor Ehrlich's 

 reaction, and is not precipitated by phosphotungstic acid. Differing from 

 the other acids, it is precipitated by basic lead acetate, and its salts are only 

 slightly soluble in alcohol. According to LIEBERMANN 3 this acid is not 

 a unit substance, and contains a part of its sulphur as ethereal sulphuric, 

 acid and it also contains uroferric acid. 



The preparation of the three above-mentioned acids is based in part 

 upon the fact that alloxyproteic acid alone is precipitated by basic lead 

 acetate and that the two other acids can be precipitated from the fil- 

 trate by mercuric acetate, the antoxyproteic acid in acetic acid solution 

 and the oxyproteic acid in neutral solution. The preparation is never- 

 theless very tedious and complicated and therefore we must refer to the 

 original works 4 for details, 



Uroferric acid is an acid isolated by THIELB 5 from the urine, according 

 to SIEGFRIED'S method for preparing pure peptone. It also contains 

 sulphur, 3.46 per cent, and has the formula C3 5 H 56 N 8 SOi 9 . The acid forms 

 a white powder which is readily soluble in water, saturated ammonium- 

 sulphate solution, and methyl alcohol. It is soluble with difficulty in 

 absolute alcohol, insoluble in benzene, chloroform, ether, and acetic 

 ether. About one half of the sulphur can be split off as sulphuric acid 

 on boiling with hydrochloric acid. The acid gives neither the biuret 

 test nor MILLON'S or ADAMKIEWICZ'S reactions. It is precipitated by 

 mercuric nitrate and sulphate, and also by phosphotungstic acid. This acid 

 is hexabasic, and its specific rotation at 18 C. (a) D = 32.5. On cleavage 

 it yields melanine substances, sulphuric acid, aspartic acid, but no hexone 



% 



1 Cloetta, Arch. f. exp. Path. u. Pharm., 40; Pregl, Pfluger's Arch., 75. 



2 Ber. d. d. chem. Gesellsch., 35. 



3 Zeitschr. f. physiol. Chem., 52. 



4 Ibid-., 46. 



5 Ibid., 37. 



