6* NUCLEAR STAINS. 



(ii.) EHBLICH'S IIJEMATOXYLIN". 



Hsematoxylin, 2 grms/; Absolute Alcohol, 100 cc. Dis- 

 solve and add Glycerine, 100 cc. ; Distilled Water, 100 cc. ; 

 Alum (Ammonia), 2 grms. ; Glacial Acetic Acid, 10 cc. 



It should be exposed to daylight for at least a month 

 before use, removing the stopper at intervals. 



(iia.) EHBLICH'S H.ZEMATOXYLIN (Ammoniated). 



Haematoxylin, 2 grms. ; Ammonium Carbonate, '4 grms.; 

 Proof Spirit, 40 cc. : dissolve the Ammonium Carbonate and 

 the Hsematoxylin in the Proof Spirit and expose to the 

 air in a shallow dish for 24 hours, make up the volume to 

 40 cc. with Proof Spirit (warming if necessary to dissolve any 

 separated crystals) and add Ammonia Alum, 2 grms., dis- 

 solved in Distilled Water, 80 cc. ; Glycerine, 100 cc. ; Rectified 

 Spirit, 80 cc. ; Glacial Acetic Acid, 10 cc. This solution re- 

 quires no " ripening" and is ready to be diluted for use (1 10). 



(iii.) DELAFIELB'S 1LZEMATOXYLIN. 



To 400 cc. of a saturated aqueous solution of Ammonia 

 Alum add 4 grms. of Haematoxylin dissolved in 25 cc. of 

 Absolute Alcohol-; leave the solution exposed to the light 

 and air in an unstoppered bottle for 3 or 4 days ; filter, and 

 add to the filtrate 10Q,cc. of Glycerine and 100 cc. of Methylic 

 Alcohol (Wood Spirit); allow the solution to stand in the 

 light- until it is a dark colour, re-filter and preserve in a 

 stoppered bottle. , , 



Ammonia Alum dissolves about 1 in 1 1 of Water. 



HJEMATOXYLIN. ,C?<P 



itebmatoxylin, 12 grms. ; Alum (Ammonia), 50 grms. ; 

 GlySRhe, 65 cc. ; Distilled Water, 130 cc. ; boil, and while 

 hot add 5 cc. of liquid Carbolic Acid. Allow the mixture to 

 stand in daylight for at least a month to ripen before using. 



I 



