NUCLEAR STAINS. 



27 



mounting unstained sections. After some time the sections 

 absorb the colour and become stained. 



(viii.) RENAUT'S EOSINATED GLYCERINE HJE- 

 MATOXYLIN. 



Concentrated aqueous solution of Eosine, 30 cc. ; saturated 

 solution of Haematoxylin in Alcohol, 40 cc. ; saturated solution 

 of Potash Alum in Glycerine, 130 cc. ; mix and let it stand 

 in a beaker for 5 or 6 weeks, so that the Alcohol may evapo- 

 rate, but protected from dust, then filter. 



If desired, the nitrate can be diluted with Glycerine. 



(ix.) HEIDENHAIN'S H2EMATOXYLIN METHOD. 



(a} Hsematoxylin, 1 grm. ; Distilled Water, 300 cc. 



(b) Chromate of Potassium, 1 grin. ; Distilled Water, 

 200 cc. Small pieces which have been hardened in Alcohol 

 or Picric Acid are placed in solution (a) for 12 to 24 hours ; 

 and then transferred for a similar time to solution (I}. Wash 

 the pieces thoroughly in Water, then dehydrate in Alcohol 

 for embedding (saturation) in Paraffin. (See p. 17.) 



This process is used for staining in bulk previous to serial 

 cutting. 



(x.) BENDA'S COFFER HJEMATOXYLIN METHOD. 



For use after hardening in Chromic Acid or Flemming's 

 Solution. Sections are placed for 24 hours in a 5 p. c. solu- 

 tion of Neutral Acetate of Copper, kept at about 40 C. (104 F.) 

 washed thoroughly in Distilled Water ; stained in a saturated 

 aqueous solution of Hsematoxylin, until the sections become 

 dark grey or blackish. Decolourise to a rather light yellow 

 with Hydrochloric Acid diluted 1 to 500 of Water. The sec- 

 tions must then be brought to a light blue colour by a second 

 immersion in the copper solution. Wash in Water, dehy- 

 drate, clear and mount in Balsam. 



