32 NUCLEAR STAINS. 



(iv.) GRENACHEK, S ALUM CARMINE. 



Carmine, 1 grm. ; Alum (Ammonia), 5 grms. ; Distilled 

 Water, 100 cc. Dissolve the Alum in the Water, add the 

 Carmine, and boil the solution for 20 minutes, allow it to cool, 

 filter, and add Distilled Water to make the volume 100 cc. 



This solution is chiefly used for the nervous system, but for 

 ordinary staining it possesses the advantage that it is nuclear 

 without after-treatment with acid. 



(v.) CZOKER'S ALUM COCHINEAL. 



Powdered Cochineal, 1 grm. ; Alum, 1 grm. ; Distilled 

 Water, 100 cc. 



Dissolve the Alum in the Water, add the Cochineal and 

 boil ; evaporate down to half of its original bulk, filter and 

 add -| cc. of Liquid Carbolic Acid to the solution. 



This is an excellent nuclear stain, and is chiefly used for the 

 central nervous system after hardening in Chromium Salts. 

 It is rather better for this purpose than Alum Carmine. 



(vi.) GRENACHER'S BORAX CARMINE. 



Carmine, 0-5 grm. ; Borax, 2 grms. ; Distilled Water, 

 100 cc. Heat the mixture to boiling point ; then add a 5 p. c. 

 solution of Acetic Acid, drop by drop, until the purple 

 colour turns red ; allow the mixture to stand for 24 hours, 

 decant, filter, and add a few drops of Carbolic Acid to preserve 

 the solution. 



About 17 cc. of the Acetic Acid will be required. 



(vii.) GRENACHER'S ALCOHOLIC BORAX CAR- 

 MINE. 



Carmine, 3 grms. ; Borax, 4 grms. ; Distilled Water 

 100 cc. Dissolve the Borax in the Water ; add the Carmine 



