NUCLEAR STAINS. 35 



given to Lee by Yignal as used in the Laboratory of Histology, 

 of the College of France. 



" Water, 1000; Picric Acid, 20; Carmine, 10; Ammonia, 50. 



' ' Put them into a stoppered bottle, and leave them for 2 or 3 

 months in a warm place ; then place the solution in a large 

 crystallizing dish and allow it to putrefy. When the liquid 

 has become reduced to four-fifths of its original bulk, remove 

 the crystals that have formed at the bottom, dry them and 

 dissolve them in a little Warm Water. Filter the solution, 

 and examine it with the microscope to see if the Carmine is 

 really dissolved ; if not, add more Ammonia and Water, and 

 repeat the above process. When the Carmine is so combined 

 as to dissolve in the warm Water, then dissolve it, evaporate 

 the solution to dryness in a stove, and reduce the Picro- 

 Carminate of Ammonia to powder. 



" For use dissolve 1 grm. of the powder in 100 cc. of Distilled 

 Water." 



(ii.) SODA FICRO-CARMINE. 



ustic Soda, 1 grm. ; Distilled Water, 1000 cc. ; dissolve 

 add Carmine, 10 grms. ; boil, filter and make up the 

 volume to 1000 cc. with Distilled Water; mix this with an 

 equal volume of Distilled Water, and add 1 p. c. aqueous 

 solution of Picric Acid so long as the turbidity, thus pro- 

 duced, continues to disappear on agitation. 



(iii.) PICRO LITHIUM CARMINE. 



Lithium Carmine solution, 100 cc. ; saturated aqueous solu- 

 tion of Picric Acid, 200300 cc. 



ANILINE NUCLEAR STAINS. 



Fuchsine (Magenta), Gentian Yiolet, Dahlia Violet, Methy- 

 lene Blue, Methyl Green, Hoffmann's Blue, Safranine, and 

 Vesuvine are indirect nuclear stains. 



