38 NUCLEAR STAINS. 



hence the names Methyl Green and Iodine Green. Now 

 Methyl Bromide or Methyl Chloride is used in its place. 



Iodine Green always contained large quantities of Violet, 

 which was most probably the cause of the amyloid reaction 

 assigned to this dye (see Methyl Green}. 



(iii.) SAFRANINE. 



Safranine, 0-5 grm. ; [Rectified Spirit, 20 cc. ; Distilled 

 Water, 80 cc. 



A good nuclear stain, and especially useful for tissues 

 that are rapidly growing and dividing. 



For nuclear staining, sections are placed in the solution for 

 5 to 10 minutes, differentiated in 90 p.c. Alcohol, dehy- 

 drated, cleared and mounted in Balsam. 



(iv.) VESUVINE (Bismarck Brown). 



Vesuvine, 0-5 grm. ; Eectified Spirit, 20 cc. ; Distilled "Water, 

 80 cc. 



A good nuclear stain, which is not easily washed out, and 

 is permanent. 



Sections are placed in this for 5 minutes, washed in 90 p.c. 

 Alcohol with or without previous treatment with water, 

 dehydrated, cleared and mounted in Balsam. 



(v.) GENTIAN VIOLET. 

 DAHLIA VIOLET. 



Gentian or Dahlia Violet, 0'5 grm. ; Eectified Spirit, 20 cc. ; 

 Distilled Water, 80 cc. 



To stain the nuclei, sections are placed in either of these 

 Violet solutions for 5 minutes, then washed in 90 p.c. Alcohol, 

 cleared and mounted in Balsam. 



Gentian and Dahlia as well as Methyl Violet are generally 

 included amongst the nuclear stains, but they are more gene- 



