40 PLASMATIC STAIKS. 



Water, then transferred to the Plasmatic stain and treated as 

 directed. 



After Hsematoxylin, other Blues, and Green, use either 

 Benzopurpurine (brownish red), Eosine (yellowish red), Ery- 

 throsine (pink), Orange, Eubin S., or Eubin and Orange ; 

 after Carmine and other Beds, use Picric Acid (yellow). 



(i.) BENZOPURPURINE. 



Benzopurpurine, 0-25 grm. ; Eectified Spirit, 20 cc. ; 

 Distilled Water, 80 cc. 



A good contrast stain to Hcematoxylin and other blue 

 nuclear stains. Sections are placed in this for 2 to 5 minutes, 

 then washed in 90 p.c. Alcohol, dehydrated, cleared and 

 mounted in Balsam. 



(ii.) EOSINE. 



Eosine (Water-soluble), 1 grm. ; Eectified Spirit, 40 cc. ; Dis- 

 tilled Water, 160 cc. 



For counterstaining, the sections are placed in this solution 

 for 2 to 5 minutes, then washed in 90 p.c. Alcohol, dehy- 

 drated, cleared and mounted in Balsam. 



There are two classes of Eosines, characterised by their more ready 

 solubility in water or in spirit. Each is useful according to the nature 

 of the mounting media to be employed. 



It is frequently stated that, when mounting in Balsam, the 

 Eosine must be dissolved in the Alcohol used for dehydrat- 

 ing to prevent the stain washing out, but this is not at all 

 necessary if the " water-soluble " variety be used. 



It is a specific stain for red blood corpuscles, which it colours 

 a copper Ted ; also for the giant cells of Leprosy and Tuber- 

 cle, staining them an orange-yellow. 



