PLASM ATIC STAINS. 41 



(iii.) ERYTHROSINE. 



Erythrosine, 1 grm. ; Rectified Spirit 40 cc. ; Distilled 

 Water, 160 cc. 



For counterstaining, sections are placed in this solution for 

 2 to 5 minutes, then washed in 90 p.c. Alcohol, dehydrated, 

 cleared and mounted in Balsam. 



(iv.) ORANGE. 



Orange, 2 grms. ; Rectified Spirit, 20 cc. ; Distilled Water, 

 80 cc. 



Sections are placed in this solution for 10 minutes, washed 

 in 90 p.c. Alcohol, dehydrated, cleared and mounted in 

 Balsam. 



(v.) PICRIC ACID (ALCOHOLIC). 



Picric Acid, 1 grm. ; 70 p.c. Alcohol, 100 cc. 



Principally used for staining fibrous tissue (skin, bone, 

 &c.). 



For counterstaining after Carmine omit the treatment with 

 Acidulated Alcohol (p. 28), and rinse the sections for a 

 couple of minutes in 70 p.c. Alcohol ; transfer to Picric Acid 

 about 5 minutes ; dehydrate in Absolute Alcohol 3 or 4 

 minutes ; clear in Cedar Oil ; mount in Balsam. 



It has been stated that Picric Acid must be added to the 

 Alcohol used for dehydrating the sections, to prevent the 

 stain washing out, but this is not necessary. 



(vi.) ACID RUBIN. 



Acid Fuchsine, 1 grm. ; Rectified Spirit, 40 cc. ; Distilled 

 Water, 160 cc. 



For counterstaining, sections are placed in this solution for 

 5 to 10 minutes, washed in 90 p.c. Alcohol, dehydrated, 

 cleared and mounted in Balsam. 



