42 TLASMATIC STAINS. 



The dyes sold under the names Acid Rubin, Acid Fuchsine, 

 and Acid Magenta have practically the same composition and 

 give the same reactions, although different samples may vary 

 somewhat in the shade of colour. 



(vii.) RUBIN AND ORANGE. 



Acid Fuchsine, 1 grm. ; Orange, 6 grms. ; Rectified Spirit, 

 60 cc. ; DistiUed Water, 240 cc. 



For counterstaining, sections are placed in this for 5 to 10 

 minutes, washed in 90 p.c. Alcohol, dehydrated, cleared and 

 mounted in Balsam. 



When the elements of the ground tissue have the same 

 affinity for each of the two colours, the result is a rich orange- 

 red; where any selective affinity exists, either of the two 

 colours will be more pronounced. 



