46 SPECIFIC STAINS. 



The uniformly round granules of these plasma cells (granu- 

 lar cells) are stained by" Aniline dyes (especially the Violets) 

 in a similar manner to bacteria, and are chiefly distinguished 

 from the latter by the fact that the granules are always 

 grouped in the form of cell-like structures, and the Aniline 

 stain is removed from the granules, but not from bacteria, by 

 treatment with a weak solution of Carbonate of Potassium. 



STAINS FOB, AMYLOID DEGENERATION. 

 METHYL VIOLET. 



Methyl Violet, 0-5 grm. ; Water, 200 cc. ; Glacial Acetic 

 Acid, 5 c.c. 



Stain sections for 20 minutes ; wash thoroughly in Distilled 

 Water, then in a mixture of equal parts Glycerine and Water 

 BO long as any colour is taken out (generally about 2 hours) 

 and finally mount in Farrant. 



The amyloid material is stained pink and the normal tissue 

 a bluish- green colour. 



When stained as usual without the addition of Acetic Acid, 

 the colour-contrast is not so striking. It may, however, be 

 improved by after-treatment with dilute acid, but it is better 

 to combine the acid with the stain. 



Most Methyl and Iodine Greens give the same reaction 

 owing to the quantity of Methyl Violet which they contain. 



IODINE. 



Iodine, 1 grm. ; Iodide of Potassium, 2 grms. ; Distilled 

 Water, 300 cc. 



Solution is most easily effected by using 10 cc. of the 

 Water to dissolve the solids, then diluting with the remainder 

 j>f the water. 



