SPECIFIC STAINS. 47 



This is the same as Gram's solution for Micro-organisms. 



An alternative formula is : 



Liquor lodi, B.P. 10 cc. ; Distilled Water, 140 cc. 



Stain the sections for 5 minutes ; wash in Water and mount 

 in thick Farrant to which a fourth of its volume of Liquor 

 lodi has been added. 



Under the microscope (a low power answers best) by trans- 

 mitted light, the section appears coloured various shades of 

 yellow, but by reflected light the amyloid substance appears 

 almost black while the normal tissue remains unaltered. 



SAFBANINE. 



Safranine, 1 grm. ; Eectified Spirit, 20 cc. ; Distilled Water, 

 80 cc. 



Stain the sections for 2 minutes and wash in water. The 

 amyloid substance gives a bright orange-red colour contrast- 

 ing well with the normal pink. 



If to be mounted, wash the sections in a mixture of equal 

 parts Glycerine and Water, frequently changed until no 

 more colour is removed (12 hours is generally sufficient), 

 then in pure Glycerine in the same way, and finally mount 

 in Farrant. 



By passing rapidly through 90 p.c. Alcohol and Absolute 

 Alcohol into Cedar Oil, the section may be mounted in Balsam, 

 but the bright orange colour of the Amyloid is always im- 

 paired by contact with spirit. 



ROSE BENGALE. 



Eose Bengale, 1 grm. ; Eectified Spirit, 20 cc. ; Distilled 

 Water, 80 cc. 



Stain the sections for five minutes, wash in 90 p.c. Alcohol, 

 dehydrate, clear and mount in Balsam. 



It demonstrates very well the commencement of amyloid 



