ft 



SPECIFIC METHODS. 51 



washed in Water, treated in the Permanganate of Potassium 

 and Pal's solution, and counter-stained with Carmine ; dehy- 

 drated, cleared, and mounted in Balsam. 



GOLGI'S SUBLIMATE METHOD. 



Small pieces (about J in. cubes) of the tissue are hardened 

 (15 to 30 days) in about 200 cc. of Muller's Fluid (p. 4) which 

 should be frequently changed. 



They are then transferred to aqueous solution of Corrosive 

 Sublimate (0-25 to 1 p.c.) which must be changed from time 

 to time as it becomes coloured by the Bichromate. 



The sublimate treatment takes from 8 to 10 days or even 

 longer. 



Sections of the material thus treated are well washed with 

 water. 



Subsequent treatment with a weak solution of Sulphide of 

 Sodium darkens the stain and makes it sharper. 





