STAINING CELLULOSE AND ITS 

 MODIFICATIONS. 



Unaltered Cellulose. This is stained by Carmine r 

 Hsematoxylin, and most of the aniline dyes. 



It is coloured blue with Chlorzine Iodine (Schulze's Solution) ;. 

 also by treatment with Iodine followed by Sulphuric Acid 

 diluted 1 to 3 of water. 



It is dissolved by Sulphuric Acid (undiluted). 



Staining with Carmine. Place a section in Grenacher's 

 Alcoholic Borax Carmine (p. 32) for 15 to 30 minutes, rinse 

 for a second in Distilled Water, transfer to 90 p. c. Alcohol ; 

 dehydrate, clear, and mount in Balsam. 



The Carmine washes out quickly in Water, therefore the 

 section must not be left there any length of time, but it is 

 better to just rinse the sections in Water, than to transfer 

 them direct from the Carmine solution to the Alcohol. 



Cellulose, nuclei, and protoplasm in general are stained. 



For staining nuclei only, see " Nuclear Staining," p. 20. 



Staining with Hsematoxylin. Kinse the sections in 

 Distilled Water for a minute or two, place them for 5 

 minutes in Ehrlich's or Delafield's Solution (p. 24) diluted 

 1 to 9 of Distilled Water; rinse in Distilled Water and transfer 

 to Tap Water (if alkaline) or to a weak solution of Bicar- 

 bonate of Sodium (1 grain to 2 ounces), until the staining 

 acquires a blue tint ; dehydrate, clear, and mount in Balsam. 



