56 



STAINING SIEVE AREAS. 



METHYL GREEN. 



Methyl Green, 0-25 grm. ; Eectified Spirit, 20 cc. ; Distilled 

 Water, 80 cc. 



PICRIC ACID (Alcoholic). 



Picric Acid, 1 grm. ; 70 p. c. Alcohol, 100 cc. 



STAINING SIEVE AEEAS. 



These are best stained by Hoffmann's Blue, but Eosine is 

 frequently employed for this purpose. They can also be 

 demonstrated by Haematoxylin, using the process given above 

 for cellulose. 



HOFFMANN'S BLUE. 



Hoffmann's Blue, l|grm. ; Eectified Spirit, 20 cc. ; Dis- 

 tilled Water, 80 cc. Glacial Acetic Acid, 0-5 cc. 



Place the sections in the stain for 5 to 10 minutes, rinse in 

 Distilled Water in which the colour is partially removed, 

 mount in Glycerine; or dehydrate, clear, and mount in Balsam. 



This is also a very good nuclear stain. 



EOSINE. 



Eosine, 2 grins ; Eectified Spir.it, 20 cc. ; Distilled Water, 

 80 cc. Place the sections in the stain for 5 to 10 minutes, 

 rinse in Water, wash in 90 p. c. Alcohol, dehydrate, clear, 

 and mount in Balsam. 



