MICRO-ORGANISMS. 59 



(i) Aqueous Solution. Methyl Violet, 1 grm. ; Eectified 

 Spirit, 20 cc. ; Distilled Water, 80 cc. 



(c) Xuhne's Solution. Methyl Yiolet, 1 grm. ; Distilled 

 Water, 90 cc. ; Alcohol, 100 cc. 



(d) * Methyl- Violet Aniline -Water. Aniline Water, 

 100 cc. ; Concentrated Alcoholic Solution of Methyl Violet, 

 11 cc. ; Absolute Alcohol, 10 cc. 



VICTORIA BLUE. 



Victoria Blue, 2 grms. ; Alcohol (50 p. c.), 100 cc. 



(vi.) FUCHSINE (Magenta). 



(a) Concentrated Alcoholic Solution. Fuchsine, 25 

 grms. ; Absolute Alcohol, 100 cc. 



(i) Aqueous Solution. Fuchsine, 1 grm.; Eectified Spirit, 

 20 cc. ; Distilled Water, 80 cc. 



(c} *Fuchsine Aniline-Water. Aniline Water, 100 cc. ; 

 Concentrated Alcoholic Solution of Fuchsine, 1 1 cc. ; Absolute 

 Alcohol, 10 cc. 



(d) Ziehl-Neelsen Solution, or Kuhne's Carbolic Fuch- 

 sine. Fuchsine, 1 grm. ; Absolute Alcohol, 10 cc. Dis- 

 solve and add 100 cc. of a 5 p. c. Aqueous Solution of Carbolic 

 Acid. 



(e] Magenta Solution (Gibbes'). Magenta, 2 grms. ; 

 Aniline Oil, 3 grms. ; Eectified Spirit, 20 cc. ; Distilled Water, 

 20 cc. 



(/) Gibbes' Double Stain. Magenta, 2 grms. ; Methy- 

 lene Blue, 1 grm. Well triturate the mixture, and then add 

 slowly Aniline Oil, 3 cc., dissolved in Eectified Spirit, 15 cc. ; 

 subsequently add Distilled Water, 15 cc., and keep stain in 

 a stoppered bottle. . 



* " ErHich-Weigert-Koch " formula. 



