MICRO-ORGANISMS. 



WITH FUCHSINE. 



Ehrlich's Method, see Tubercle Bacilli, p. 68. 



Kuhne's Methods. 



Method of Staining Bacteria not belonging to the Group of 

 Tubercle Bacilli in the Tissues with Fuchsine (Kuhne]. 



The bacilli of mouse septicaemia, anthrax, and other 

 bacilli, and several kinds of cocci may be stained by the 

 following method, which will not apply to the bacilli of 

 glanders and typhoid fever : 



Sections are first dehydrated in Alcohol and then stained 

 from 3 to 5 minutes in Carbolic Fuchsine, rinsed in water, 

 dipped for 1 minute into Alcohol, and extracted in Methyl 

 Green Aniline Oil, which requires from 1 5 minutes to 2 hours, 

 according to the thickness of the sections. The sections are 

 then passed through Ethereal Oil and Xylol. 



Method of Staining with Black Brown and Fuchsine (Kuhne}. 



Anthrax sections are stained for 5 minutes in Carbolic 

 Black Brown, rinsed in Lithia Water (p. 61), and then in 90 

 p. c. Alcohol ; the sections are placed for 5 minutes in Car- 

 bolic Fuchsine, and decolourised in Fluorescine Alcohol. 



In this method Carbolic Black Brown acts as a mordant to 

 Fuchsine, so that the Anthrax bacilli do not lose the red 

 colour in the Fluorescine Alcohol. 



