70 MICRO-ORGANISMS. 



bulk of pure Aniline Oil ; then pass into Ethereal Oil for 

 2 minutes, afterwards into Xylol, and mount in Balsam. 



Gibbes' Method of Double Staining. 

 Cover-glass preparations are placed for 4 minutes in Gibbes' 

 double stain, which has been previously slightly heated; 

 sections should be placed in the stain at the ordinary tem- 

 perature for some hours. The cover-glass preparations or 

 sections are then washed in Methylated Spirit till no more 

 colour comes away, dehydrated, cleared in Cedar Oil and 

 mounted in Balsam. 



LEPROSY BACILLUS. 



The same staining methods may be employed as for the 

 tubercle bacillus. 



As leprosy bacilli stain much more quickly in the Fuchsine, 

 the following plan has been suggested for distinguishing 

 them : 



Sections are stained for 1 to 3 minutes in the Aqueous Solu- 

 tion of Fuchsine (p. 59) ; then placed for 30 seconds in a 

 solution consisting of 90 p. c. Alcohol, 10 parts; Nitric Acid 

 (Sp. g. 1 '420), 1 part ; dehydrate, clear, and mount in Balsam. 



Treated in this way the leprosy bacilli appear well stained, 

 while the tubercle bacilli are not stained at all. 



ANTHRAX BACILLUS. (MILZBRAND BACILLUS.) 



This may be stained with Yiolet by Gram's method 

 (p. 64) or one of the modifications ; with Fuchsine or Methy- 

 lene Blue by Kuhne's methods (pp. 66 and 67). 



GLANDERS BACILLUS. (ROTZ BACILLUS. 

 BACILLUS MALLEI.) 



Cover-glass preparations are stained with Methylene Blue 

 by Kuhne's method (p. 66) or by Loffler's method (p. 65). 



