72 MICRO-ORGANISMS. 



Aniline-Water, are rinsed in Alcohol ; placed in Gram's Solu- 

 tion (p. 6 1 ) rinsed in Alcohol, cleared and mounted. The micro- 

 cocci become a dense blue on a faintly yellow ground substance. 



Friedlaender's Methods 



For staining the capsule as well as the cocci. 



Cover-glass Preparations. 



Cover-glass preparations of the sputum are treated for 3 

 minutes with a 1 p. c. solution of Acetic Acid. The excess of 

 Acid is then removed by filter-paper and the cover-glass is 

 allowed to dry ; the preparations are next placed in Gentian - 

 Violet Aniline-Water (p. 58) for half a minute; then washed 

 in Water, dried, and mounted in Balsam. 



For Tissues the following Plan is used: 



Sections are placed for 24 hours in the following solution in 

 a warm place : Concentrated Alcoholic solution of Gentian 

 Violet, 50 cc. ; Distilled Water, 100 cc. ; Glacial Acetic Acid, 

 10 cc. They are then placed from 1 to 2 minutes in 0-1 p. c. 

 Acetic Acid ; dehydrated, cleared, and mounted in Balsam. 



BACILLUS OF DIPHTHERIA. 



To stain these Lomer used his process, described p. 65. 

 They may also be stained by Gram's method (p. 64). 

 A piece of the false membrane is placed on a cover-glass 

 and treated as a cover-glass preparation. 



BACILLUS OF ENTERIC FEVER. 



Gaffky's Method. 



The organ from which the sections are made must be 

 hardened in Alcohol. 



The sections are placed for 20 to 24 hours in a deep blue 

 opaque solution, which must be freshly made by adding a 



