MICRO-ORGANISMS. 73 



saturated Alcoholic solution of Methylene Blue to Distilled 

 Water ; they are then washed in Distilled Water (not acidu- 

 lated), thoroughly dehydrated in Absolute Alcohol, cleared in 

 Oil of Turpentine, and mounted in Balsam. 



These may also be stained by Loffler's Method (p. 65). 



It is usually stated that Gram's Method is not applicable 

 as it removes the stain from the bacilli, but Woodhead affirms 

 that if the sections containing these bacilli are allowed to 

 remain for 10 minutes in a 3- p. c. solution of corrosive sub- 

 limate, and then stained by Gram's method the bacilli are 

 deeply coloured. 



ACTINOMYCOSIS. 



(i.) Weigert's Method. 



Sections are immersed for 1 hour m Orseille (p. 60) ; they 

 are quickly rinsed with Alcohol and counterstained with Gen- 

 tian Yiolet. 



If it is desired to stain the mycelium also, the sections, after 

 being stained with Orseille, are submitted to Weigert's modi- 

 fication of Gram's method (p. 64). 



(ii.) Plant's Method. 



Sections are placed for 10 minutes in Gibbes' Magenta solu- 

 tion (p. 59) (or Carbolic Fuchsine) at 45 C. ; next they are 

 rinsed in Water and placed in saturated aqueous solution of 

 Picric Acid mixed with an equal volume of Absolute Alcohol 

 for 5 to 10 minutes ; they are then washed in Water, passed 

 through 50 p. c. Alcohol into Absolute Alcohol, cleared in 

 Cedar Oil, and mounted in Balsam. 



(iii.) Modification of Plant's Method. 



Sections are placed for 10 minutes in Carbolic Fuchsine 

 (p. 59) and decolourised for 24 hours in Fluorescine Alcohol. 



