74 MICRO-ORGANISMS. 



Stain the nuclei with Ehrlich's Hsematoxylin (p. 24) and 

 counterstain faintly with Benzopurpurine (p. 40). 



(iv.) Babes' Method. 



Stain sections in Babes' Safranine solution (p. 60) for 2 

 minutes, rinse in Alcohol, and decolourise in Gram's solution 

 (p. 61). 



Only the clubs remain stained. 



MAKING COVER GLASS PREPARATIONS. 



To thoroughly clean the cover-glass before use, wash it 

 in Solution of Potash, rinse it in Diluted Nitric Acid, and 

 afterwards in Alcohol. 



In the case of blood, urine, and fluid discharges, a little is 

 taken upon the point of a platinum needle which has been 

 previously heated to redness, spread out upon the clean cover- 

 glass, allowed to dry, and the cover-glass passed three times 

 through the flame of a spirit-lamp to coagulate the Albumen. 



In the case of sputum it should be rubbed to and fro over 

 the cover-glass with a glass needle (thin glass rod drawn out 

 to a point) until a uniformly thin layer of mucus is formed on 

 the cover-glass. It is then allowed to dry ; the drying can if 

 necessary be accelerated by a gentle heat, and in the case of 

 tough sputum a vertical stream of air from an air-ball syringe 

 is useful. When dry the preparation is passed three times 

 through the flame as above. 



When examining tubercular sputum, the yellow caseous 

 masses should be selected. 



MAKING COVER-GLASS IMPRESSIONS. 



A perfectly clean cover-glass is placed upon the culture, 

 gently pressed, and then carefully raised. It is allowed to 

 dry, passed three times through the flame of a spirit-lamp, 

 stained by one of the methods, and mounted in Balsam. 



