76 MICRO-ORGANISMS. 



STAINING SPORES. 



If the cover-glass preparation be heated for much longer 

 than in the ordinary method for bacilli, the spores will be 

 stained as readily as the bacilli. 



After a cover-glass preparation is made, it is passed several 

 times (12) through the flame, or heated to a temperature of 

 210 F. for half an hour, or exposed to the action of strong 

 Sulphuric Acid for a few seconds ; then stained with aqueous 

 solution of Fuchsine, Methylene Blue, or Gentian Violet. 



DOUBLE-STAINING SPORE-BEARING BACILLI. 

 Neisser's Method. 



Cover-glass preparations treated in the ordinary way (not 

 specially heated as in the foregoing method) are immersed 

 for 20 minutes in the Fuchsine Aniline- Water (p. 59) heated 

 to 80 or 90 C. (176 or 194 F.) ; the cover-glass is then 

 rinsed in Water, Alcohol, or weak Acid, according to the 

 nature of the bacilli ; counterstained with aqueous solution of 

 Methylene Blue, rinsed in Water, dried, and mounted in 

 Balsam. 



The spores are stained red, and the other part of the bacilli 

 blue. 



STAINING PLAGELLA. 



(i.) Koch's Method. 



Cover-glass preparations are immersed in 1 p. c. aqueous 

 solution of Hsematoxylin ; they are then transferred to a 5 

 p. c. solution of Chromic Acid, or to Miiller's Fluid, dried 

 and mounted in Balsam. 



The flagella are stained a brownish black. 



