MICRO-ORGANISMS. 77 



(ii.) Crookshank's Method. 



Cover-glass preparations are stained with a drop of concen- 

 trated alcoholic solution of Gentian Violet ; the cover-glass 

 is then rinsed in Water, allowed to dry, and mounted in 

 Balsam. 



(iii.) Loffler's Method. 



The stock solution is made by adding to 10 cc. of a 25 p. c. 

 Aqueous Solution of Tannin, 5 cc. of cold saturated Aqueous 

 Solution of Ferrous Sulphate, and 1 cc. of Aqueous or Alco- 

 holic Solution of Fuchsine or Methyl Yiolet. 



As the treatment, however, varies with the different micro- 

 organisms, the reader is referred to an abstract of the paper 

 in Jour. R.M.S., 1890, p. 678. 



