84 MOUNTING MEDIA. 



DAMMAR AND MASTIC. 



Gum Dammar, 100 grms. ; Oil of Turpentine, 200 cc. Dis- 

 solve and filter. Gum Mastic, 50 grms. ; Chloroform, 200 cc. 

 Mix the two solutions. 



This is an old formula which is still used by some workers. 



GLYCERINE. 



Two or three volumes of Glycerine to one of Distilled Water 

 is a good medium in which to examine tissues transferred 

 from aqueous fluids. For mounting permanently, the objects 

 should be immersed in the above diluted Glycerine, which 

 may be increased in strength if desirable ; delicate objects 

 may be placed in a mixture of Glycerine, 1 ; Alcohol, 1 ; 

 Water, 1 ; which is then allowed to concentrate by evapo- 

 ration. 



GLYCERINE JELLY. 



Immerse 100 grms. of French Gelatine in Chloroform 

 .Water, and, when soft, drain away the excess of water. 

 Dissolve the softened Gelatine in 750 grammes of Glycerine 

 over a water-bath, and add 400 grammes of Chloroform 

 Water with which has been incorporated about 50 grammes 

 of fresh egg albumen, and mix thoroughly. Heat the mix- 

 ture to boiling for about five minutes to coagulate the albu- 

 men; make up the total weight to 1,550 grammes with 

 Chloroform Water. Filter in a warm chamber, but pro- 

 tected as much as possible from evaporation. 



Tissues can be mounted in this direct from water, but it is 

 better to immerse them for a little while in equal parts of 

 Glycerine and Distilled Water. Place them on a slide ; re- 

 move superfluous fluid, and apply a few drops of the melted 



