a 97 % chance of detecting as little as 1% hybridization between westslope cutthroat trout 

 and rainbow trout. 



This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of twenty- four, we have an 

 S5% chance of detecting as little as 1% hybridization between Yellowstone cutthroat 

 trout and westslope cutthroat trout. 



Dunham Creek: All thirty successfully amplified individuals in this sample displayed 

 only PINE fragments diagnostic of Westslope cutthroat trout. With a sample size of 

 thirty, we have a 99 % chance of detecting as little as 1% hybridization between 

 westslope cutthroat trout and rainbow trout. 



This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of thirty, we have a 91% 

 chance of detecting as little as 1% hybridization between Yellowstone cutthroat trout and 

 westslope cutthroat trout. 



Rock Creek: All twenty-five successfully amplified individuals in this sample displayed 

 only PINE fragments diagnostic of Westslope cutthroat trout. With a sample size of 

 twenty-five, we have a 97 % chance of detecting as little as 1% hybridization between 

 westslope cutthroat trout and rainbow trout. 



This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of twenty- five, we have an 

 87% chance of detecting as little as 1% hybridization between Yellowstone cutthroat 

 trout and westslope cutthroat trout. 



Second Creek: All twenty-seven successfully amplified individuals in this sample 

 displayed only PINE fragments diagnostic of Westslope cutthroat trout. With a sample 

 size of twenty-seven, we have a 98 % chance of detecting as little as 1% hybridization 

 between westslope cutthroat trout and rainbow trout. 



This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of twenty-seven, we have an 

 89% chance of detecting as little as 1% hybridization between Yellowstone cutthroat 

 trout and westslope cutthroat trout. 



Smith Creek: All twenty-eight successfully aniplified individuals in this sample 

 displayed PINE fragments diagnostic of Westslope cuttliroat trout. One individual also 

 displayed PINE fragments diagnostic of rainbow trout. With a sample size of twenty- 

 eight, we have a 98 % chance of detecting as little as 1% hybridization between 

 westslope cutthroat trout and rainbow trout. 



This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of twenty-eight, we have an 

 87% chance of detecting as little as 1% hybridization between Yellowstone cutthroat 

 trout and westslope cutthroat trout. 



