Spring Creek: All twenty-seven successfully amplified individuals in this sample 

 displayed PINE fragments diagnostic of Westslope cutthroat trout. Eleven individuals 

 also displayed PINE fragments diagnostic of rainbow trout. With a sample size of twenty- 

 seven, we have a 98% chance of detecting as little as 1% hybridization between 

 westslope cuttliroat trout and rainbow trout. 



This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of twenty-seven, we have an 

 89% chance of detecting as little as 1% hybridization between Yellowstone cutthroat 

 trout and westslope cutthroat trout. 



Twelvemile Creek: All fifteen successfully amplified individuals in this sample 

 displayed PINE fragments diagnostic of Westslope cutthroat trout. One individual also 

 displayed PINE fragments diagnostic of rainbow trout. With a sample size of fifteen, we 

 have an 88%) chance of detecting as little as 1% hybridization between westslope 

 cutthroat trout and rainbow trout. 



This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of fifteen, we have a 10% 

 chance of detecting as little as 1%) hybridization between Yellowstone cutthroat trout and 

 westslope cutthroat trout. 



Washoe Creek: All twenty-eight successfully amplified individuals in this sample 

 displayed PfNE fragments diagnostic of Westslope cuttliroat trout. Four individuals also 

 displayed PfNE fragments diagnostic of rainbow trout. With a sample size of twenty- 

 eight, we have a 98%) chance of detecting as little as l%o hybridization between westslope 

 cutthroat trout and rainbow trout. 



This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of twenty-eight, we have an 

 89%) chance of detecting as little as 1% hybridization between Yellowstone cutthroat 

 trout and westslope cutthroat trout. 



Blanchard Creek: All twenty-seven successfully amplified individuals in this sample 

 displayed PINE fragments diagnostic of Westslope cutthroat trout. Seventeen individuals 

 also displayed PINE fragments diagnostic of rainbow trout. With a sample size of 

 twenty-seven, we have a 98%) chance of detecting as little as 1% hybridization between 

 westslope cutthroat trout and rainbow trout. 



This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of twenty-seven, we have an 

 89% chance of detecting as little as 1% hybridization between Yellowstone cutthroat 

 trout and westslope cutthroat trout. 



Dick Creek: All twenty-seven successfully amplified individuals in this sample 

 displayed only PINE fragments diagnostic of Westslope cutthroat trout. With a sample 

 size of twenty-seven, we have a 98% chance of detecting as little as 1% hybridization 

 between westslope cutthroat trout and rainbow trout. 



