This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of twenty-seven, we have an 

 89% chance of detecting as little as \% hybridization between Yellowstone cutthroat 

 trout and weslslope cutthroat trout. 



Little Fish Creek: All twenty-seven successfully amplified individuals in this sample 

 displayed only PINE fragments diagnostic of Westslope cutthroat trout. With a sample 

 size of twenty-seven, we have a 98% chance of detecting as little as 1% hybridization 

 between westslope cutthroat trout and rainbow trout. 



This sample was also tested for introgression with Yellowstone cuttliroal trout. No 

 evidence of introgression was detected. With a sample size of twenty-seven, we have an 

 89% chance of detecting as little as 1% hybridization between Yellowstone cutthroat 

 trout and westslope cutthroat trout. 



Montiire Creek: All twenty-seven successfully amplified individuals in this sample 

 displayed PINE fragments diagnostic of Westslope cutthroat trout. Four indi\iduals also 

 displayed PINE fragments diagnostic of rainbow trout. With a sample size of twenty- 

 seven, we have a 98% chance of detecting as little as 1% hybridization between 

 westslope cutthroat trout and rainbow trout. 



This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of twenty-seven, we have an 

 89% chance of detecting as little as 1% hybridization between Yellowstone cutthroat 

 trout and westslope cutthroat trout. 



Shanley Creek: All twenty-seven successfully amplified individuals in this sample 

 displayed PINE fragments diagnostic of Westslope cutthroat trout. One individual also 

 displayed PINE fragments diagnostic of rainbow trout. With a sample size of twenty- 

 seven, we have a 98% chance of detecting as little as 1% hybridization between 

 westslope cutthroat trout and rainbow trout. 



This sample was also tested for introgression with Yellowstone cutthroat trout. No 

 evidence of introgression was detected. With a sample size of twenty-seven, we have an 

 89% chance of detecting as little as 1% hybridization between Yellowstone cuttliroat 

 trout and westslope cutthroat trout. 



Sincerely, 



.Aaron E. Martin 



Cc: Ron Pierce (electronic copy) 



