Wild Trout and Salmon Genetics Laboratory 



Division of Bioloi^kal Sciences * University oj Montana * Missoula, MT5'JS12 

 (406)243-5503/6749 Fax (406)243-4184 



March 17.2003 



LADD KNOTEK 

 Genetics Contact, Region 2 

 iVIT Fish, Wildlife, and Parks 

 3201 SpurginRd 

 Missoula, MT 59801 



Dear LADD: 



We have completed analysis of the following samples: 



Table 1. Summary of results. 



Sample Water Name/Location/Collection Date/ 



Collector 



N # markers Species ID 



Power (%) % WCT Individuals 



Chimne.v Creek 



12N i:\V24 



7/1 1/00 



LADD KNOTEK. 



9 6 WCT 



100 



Twelvemile Creek 



19N28VV23 



7/21,00 



LADD KNOTEK 



Skalkaho Cr, Ward Ditch 



05N20VV16 



9/10/02 



CHRIS CLANCY 



17 6 WCTXRBT 87 



25 6 WCTXRBT 95 



98.7 2 



"Number of samples successfully analyzed (average, as some individuals do not amplify for all marker loci). " 



Number of markers analyzed that are diagnostic for the non-native species. 

 "■'Codes: WSCT = vvestslope cutthroat trout (Oncorhynchus clarki lewin); RBT= rainbow trout (O /nvkiss): YSCT= Yellowstone cutthroat trout 

 (O cUirki bouvien). Only one species code is listed when the entire sample possessed alleles from that species only However, it must be noted 

 that in such cases we cannot detlnitisely rule out the possibility that some or all of the individuals are hybrids; we merely have not detected an\ 

 non-native alleles at the limited number of loci examined (see Power "o column). Species codes separated by "x" indicate hybridization between 

 those species. 



' Number corresponds to the percent chance we have to detect \% hybridization given the sample size and number of diagnostic markers used 

 (e.g.. li individuals are reciuired to yield a ')5°o chance to detect 1% hybridization of rainbow or Yellowstone cutthroat trout into a westslope 

 trout population). 



Miulicaies the genetic contribution of westslope cutthroat trout to the sample assuming Hardy-Weinberg proportions. This number is reported 

 only it samples appear to come from a random mating population and can be analyzed at the population level 



Indicates number of individuals with genotypes corresponding to the species code column when the sample can be analyzed on the indiv idual 

 level only; this occurs when alleles are not randomly distributed and hybridization appears to be recent and/or if the sample appears to consist ^^f 

 an admixture of populations. 

 Note: For further details on each sample, see the "Sample Details" section below 



