Wild Trout and Salmon Genetics Laboratory 



Division of Biological Sciences * i'niversity of Montana ' Missoula, MT 59812 

 (406)243-5503/6749 Fa.x (4116)243-41 H4 



March 14. 2003 



Ron Pierce 

 R-2 Keadquaners 

 3201 Spurgin Road 

 Missoula, MT 59804 



Ron: 



We have completed analysis of the following samples: 



Reach 1 (n=7): Code 



North Fork to Nevada Creek 17-21 tluough 17-30 



Reach 2 (n=9): 18-11 through 1 8-20 



Nevada Creek to Arrastra Creek 



Reach 3 (n=9): 1 9-0 1 through 1 9-09 



Arrastra Creek to Lincoln 



Each individual from the above samples showed only PINE fragments diagnostic of 

 westslope cutthroat trout. None of the twenty-five individuals exhibited markers 

 characteristic of rainbow trout. We cannot be sure, however that these indi\'iduals did not 

 come from a hybrid population. Due to random reshuffling of alleles during sexual 

 reproduction, many hybrid individuals could appear "pure"' because of the limited 

 number of marker loci used. 



Sincerely, 



Margaret K. Cook 



