Douglas Creek (8/20/01) 

 2257 Flint Creek 



T9N R13WSec23 



24 



WSCT 



85 



94 



100 



Hogback Creek (8/1 7/99) 



78 



90 



75 



70 



"Number of samples analyzed; if combined with previous sample (indicated in "Location" column), 

 number indicates the combined sample size; if present, the number in (; is the average number successfully 

 analyzed per locus (some individuals do not amplify for all marker loci). 

 "Tvlumber of markers analyzed that are diagnostic for the non-native species. 



'Codes: WSCT = westslope cutthroat trout (Oncorhynchus clarki lewisi); RBT= rainbow trout (O. mykiss); 

 YSCT= Yellowstone cutthroat trout {0. clarki bouvieri); Bull Trout (Salvellniis confluentus). Only one 

 species code is listed when the entire sample possessed alleles from that species only. However, it must be 

 noted that in such cases we cannot defmitively rule out the possibility that some or all of the individuals are 

 hybrids; we merely have not detected any non-native alleles at the limited number of loci examined (see 

 Power % column). Species codes separated by "x" indicate hybridization between those species. 

 ''Number corresponds to the percent chance we have to detect 1% hybridization given the number of 

 individuals successfully analyzed and the number of diagnostic markers used (e.g., 25 individuals are 

 required to yield a 95% chance to detect 1% hybridization of rainbow or Yellowstone cutthroat trout into a 

 westslope trout population using the 6 available markers). Not reported when hybridization is detected. 

 "Indicates the genetic contribution of bull trout to the sample assuming Hardy-Weinberg proportions. Tliis 

 number is reported only if samples appear to come from a randomly mating population and can be analyzed 

 at the population level. 



^Indicates number of individuals with genotypes corresponding to the species code column when the 

 sample can be analyzed on the individual level only; this occurs when alleles are not randomly distributed 

 and hybridization appears to be recent and/or if the sample appears to consist of an admixture of 

 populations. 

 *See the "Sample Details" section below. 



Brief Description of Methods: 



Polymerase chain reaction (PCR) ampUfication of paired interspersed nuclear DNA 

 elements (PINEs) was used to determine each fish's genetic characteristics at multiple 

 regions of the nuclear DNA. This method produces DNA fragments that can be used to 

 distinguish between various cuttliroat trout subspecies {Oncorhynchus clarki spp.), 

 rainbow trout (O. mykiss) and their hybrids, and between bull trout {Salvelinus 

 confluentus), brook trout (S. fontinalis), and their hybrids. The presence^of a PINE 

 marker is dominant to absence. First-generation (F|) hybrids will have all the diagnostic 

 markers characteristic of the two hybridizing species. Backcrossed individuals will 

 possess some, but not all, markers characteristic of both parental species. The appearance 

 of a marker indicates the individual is either heterozygous or homozygous for that 

 marker, which precludes us from directly calculating allele frequencies. 



