Wild Trout and Salmon Genetics Laboratory 



I)n ision of liioloiiical Sciences * L'niversity of Molilalia * Missoula, MT 59812 

 (406) 243-5503/6 749 Fax (406) 243-41 84 



March 23, 2004 



Ron Pierce 



Genetics Contact, Region 2 

 Montana Fish, Wildhfe & Parks 

 3201 Spurgin Road 

 Missoula, MT 59801 



Ron: 



We have completed analysis of the following samples submitted by yourself and Ron 

 Pierce, under the Montana Fish, Wildlife and Parks budget for Region 2: 



Table 1. Summarv of results 



Sample Site Name, Collection Date, 

 # Biologist 



Location 



N" markers'" Population ID' Power (%)'' % Westslope' Individuals 

 YSCT RBT YSCT RBT 



1 column), ■^ 



Blackfoot Telemetry Samples 



06/16/03 20 4 7 WSCTxRBT 



Pierce 

 "Number of samples successfully analyzed; if combined with previous sample (indicated in "Location" 

 number indicates the combined sample size; if present, the number in () is the average number successfully analyzed 

 per locus (some individuals do not amplify for all marker loci). 

 ""Number of markers analyzed that are diagnostic for the non-native species. 



'Codes: WSCT = westslope cutthroat trout (Oncorhynchus clarki lewisi); RBT= rainbow trout (O. mykiss); YSCT= 

 Yellowstone cutthroat trout (O clarki boiivieri). Only one taxon code is listed when the entire sample possessed alleles 

 from only that taxon. However, it should be noted that in such cases we cannot completely rule out the possibility that 

 some or all of the individuals are hybrids; we merely have not detected any non-native alleles at the limited number of 

 loci examined (see Power % column). Codes separated by "x" indicate hybridization between the taxa. 

 ''Number corresponds to the percent chance we have to detect 1% hybridization given the number of individuals 

 successfully analyzed and the number of diagnostic markers used (e.g., 25 individuals are required to yield a 95% 

 chance to detect 1% hybridization of rainbow or Yellowstone cutthroat trout into a westslope trout population using 6 

 markers). Not reported when hybridization is detected. 



'Indicates the genetic contribution of westslope cutthroat trout to the sample assuming Hardy- Weinberg proportions. 

 This number is reported only if the sample appears to come from a random mating population. 

 Indicates number of individuals with genotypes corresponding to the taxon in the code column when the sample does 

 not appear to have come from a random mating hybrid swarm. 

 'See the "Sample Details" section below. 





Brief Description of Methods: 



Polymerase chain reaction (PCR) amplification of paired interspersed nuclear DNA 

 elements (PlNEs) was used to detennine each fish's genetic characteristics at multiple 

 regions of the nuclear DNA. This method produces DNA fragments that can be used to 

 distinguish between various cutthroat trout subspecies (Oncorhynchus clarki spp.), 

 rainbow trout {O. mykiss) and their hybrids, and between bull trout (Salvelinus 

 confluentus), brook trout {S. fontinalis), and their hybrids. The presence of a PINE 



