Due to the random reshuffling of alleles during sexual reproduction and the limited 

 number of diagnostic markers we use, we cannot be sure that the individuals displaying 

 only westslope cutthroat PINE fragments did not come from a hybrid population or that 

 they are truly "pure." As a main stem sample, this analysis does not represent a single 

 population, and further analysis is not possible. 



The original letter also asked us to provide hybridization information for each individual 

 fish, labeled with a four-digit code that corresponded to a transmitter number. This 

 numbers was kept throughout the analysis. I have attached a hybridization matrix of your 

 samples along with this summary report. 



Please call the lab if you have any questions. 



Sincerely, 



Aaron E. Martin 



Cc: Ladd Knotek (electronic copy) 



