44 METHODS OF MICEOSCOPICAL RESEARCH. 



striped muscle ; heart and pericardium of a small 

 animal ; small arteries and capillaries from the 

 brain of a sheep, after scraping away the brain- 

 substance ; middle-sized artery such as metacarpal 

 of the horse; trachea and lungs. These are gently 

 injected and then immersed in the fluid. 



The lips, tongue, salivary glands, tonsils, ceso- 

 phagus (distended and tied), stomach (after washing 

 away its contents with f per cent, salt solution), 

 small and large intestines, liver in half-inch cubes, 

 ureter and bladder (distended), ovary, fallopian 

 tubes, uterus (distended per vaginam) may be 

 placed in the solution. 



Besides the above, which may be taken from 

 either a dog, cat, or guinea pig, the following 

 should be obtained: The thymus gland of an 

 infant; skin of scalp, finger and palm of hand, 

 sole of foot from the human subject; also a nail. 

 The eye of an ox divided transversely just behind 

 the cornea for the ciliary muscle, sclerotic, cornea, 

 and iris; also the choroid and retina. Of course 

 both halves have to be used. The prostate gland 

 and penis of a guinea pig. The cervix uteri of 

 a cow. Mammary gland of an animal near the 

 full period of gestation. The placenta of a cat, 

 or guinea pig. The umbilical cord, which must 

 be cut into pieces an inch long, and hardened for 

 two days in Miiller's fluid before being placed in 

 the present medium. 



All the above tissues must be daily examined 

 after the third day, and each transferred to spirit 

 after it has become tough. Moreover, the fluid 

 should be changed after the first twenty-four 

 hours, and at intervals of a few days afterwards. 



