48 METHODS OF MICROSCOPICAL RESEARCH. 



olfactory epithelium, cochlea, testis, epididymis 

 and vas-deferens, ovary, human placenta, &c. 



Muller's Fluid and Spirit. 



Take three parts of Muller's fluid and add to it 

 one part of methylated spirit and keep it in a dark 

 place. The mixture should be made only as re- 

 quired. 



This is especially useful for the central nervous 

 system. The removal of the brain and spinal cord 

 without injury may here be described. Immediately 

 after death the skin is removed, or at least the skin 

 over the back and neck. Then separate the neck 

 and head from the trunk about the middle of the 

 neck. Next clear away the muscles on each side of 

 the vertebral spines and clip away every spine as 

 close as possible with scissors or bone forceps. 

 Next with ordinary forceps grasp the laminaB, which 

 cover over the spinal cord, one lamina at a. time, 

 and break it outwards by inserting one blade of the 

 forceps wiihin the neural canal, the other on the 

 upper surface of the lamina. Now advance down 

 the spine, breaking the laminse outwards right and 

 left till all the cord with its membranes is exposed. 

 The head portion is dealt with in like manner by 

 breaking off, bit by bit, the top of the skull, insert- 

 ing one blade into the interior, of course, after 

 clearing away the skin and muscles. 



The spinal cord and membranes of a cat, dog, or 

 rabbit should be carefully taken out and suspended 

 in a deep, narrow vessel. The fluid should be 

 changed at the end of twenty-four hours, then after 

 a week. At the end of this time, divide into pieces 



