THE PREPARATION OF ANIMAL TISSUES. 53 



quantity of glycerine and water for a fortnight ; 

 stain with weak magenta solution, " tease " in 

 glycerine, and cover (Stirling). 



Miscellaneous Preparations. 

 Salt Solution. 



This is made as a f per cent, solution, that 

 is, 75 grm. in 100 c.c. of water. It is used for 

 washing away foreign matter from organs before 

 immersing them in other fluids, and for examining 

 fresh tissues. In so using it, a very small drop is 

 placed on a slide, and in it is immersed a minute 

 piece of the structure about to be examined. 

 "Tease" this out with needles; then put over it 

 a cover glass. 



The following are to be studied : Fresh columnar 

 epithelium from the small intestines ; ciliated epithe- 

 lium scraped from the roof of the frog's mouth; 

 ciliary motion may be seen in the yellowish coloured 

 gills of the common salt water mussel. Ligamen- 

 tum nuchse of ox ; subcutaneous connective tissue ; 

 adipose tissue ; red marrow from a long bone ; 

 striped muscle (best seen in sartorius of the frog) ; 

 nerve fibre, sciatic of the frog ; fresh pia mater ; 

 fresh spleen (the ox spleen by preference) ; thymus 

 gland ; kidney ; Gasserian ganglion ; placenta ; 

 decidua, &c., may all be so treated. 



Glycerine. 



Margarine crystals may be obtained by steeping 

 morsels of fat for twenty-four hours in glycerine. 

 These post-mortem products appear as delicate 

 needles. 



