58 METHODS OF MICROSCOPICAL RESEARCH. 



Membranous Connective Tissue. The omenta of 

 an adult cat and rabbit, also of a young rabbit, are 

 to be treated like the omentum of rabbit as already 

 described, to show the membranous connective 

 tissue. The omentum of the young rabbit shows 

 developing fat cells and blood vessels. Some of the 

 above should be stained with logwood. 



Adenoid Tissue. "With a hypodermic syringe in- 

 ject a fresh lymphatic gland with a J per cent, 

 solution, and after placing it for twenty-four hours 

 in alcohol, make sections. These are stained with 

 logwood, and exposed to light till brownish. 



Cement Substance of Non-striped Muscle. Washout 

 a small length of intestine of a rabbit with distilled 

 water ; then fill it with a per cent, solution, and 

 tie both ends ; then place it in a J per cent, solution 

 for a quarter of an hour. Wash away all the silver, 

 and cover over with water in a saucer. Thin 

 Iamina3 of the outer muscular fibres are to be 

 stripped off with broad nibbed forceps. Mount 

 some stained with logwood in balsam ; others un- 

 stained, as desired. These also show the lymphatics. 



Sciatic Nerve of a Frog. To show Ranvier's- 

 crosses, kill a frog, and dissect out the sciatic nerve, 

 wash it in distilled water, then place a piece a line 

 in length in a J per cent, solution for five minutes. 

 Wash it now thoroughly in water and tease it care- 

 fully in glycerine, cover, and expose to light till 

 brownish. Mount a small length of an entire thick- 

 ness of an intercostal nerve of a rat or mouse, to 

 show its endothelial covering. Of course it is not 

 to be teased, and it must be very carefully washed 

 or the endothelium will be destroyed. 



Lymphatics of the Diaphragm. Expose the 



