60 METHODS OF MICROSCOPICAL RESEARCH. 



A Piece of Skin. This should be taken from the 

 snout of a pig or a mole, or both ; also the soft 

 part of a duck's bill. Sections show the nerves of 

 skin. 



Striped Muscle. The recti muscles of a rabbit's 

 eye are to be taken, a strip cut off lengthwise. 

 These show nerve terminations in striped muscle. 



Muscle ivith its Tendon. Take a piece of the 

 diaphragm of a rabbit ; let the piece be part of the 

 centrum tendinum with its attached muscle. Sec- 

 tions made in the long axis of the muscle fibres 

 show the terminations of muscle in tendon; that 

 is to say, the connecting links are shown. 



Tendon from the Tail of a Mouse. After treating 

 a few leashes as above, snip off a piece a line in 

 length; tease in glycerine and cover, or simply 

 press the cover glass upon it till it flattens out. 

 This preparation shows the relations of the cells 

 and fibres in tendon. 



Tail of Rat. After treating as above, with the 

 exception that it must remain an hour in the gold 

 solution, decalcify, by placing in chromic and nitric 

 solution, then make transverse sections and stain 

 with logwood. 



Nerve Ganglia. Treat the heart of a frog, in 

 small pieces, as above ; dissect out the nerves and 

 ganglia. These are also found lying along the 

 course of the abdominal aorta of the frog, and may 

 be taken from there if preferred. The nerve 

 ganglia of the bladder and ureter of a small mammal 

 may likewise be thus demonstrated. 



