66 METHODS OF MICROSCOPICAL RESEARCH. 



the ammonia has evaporated. Dissolve a drachm 

 and a half of best gelatine in a drachm and three- 

 quarters of water ; add a few drops of glacial acetic 

 acid. This fluid is to be injected warm. 



ACID CARMINE FLUID. (Dr. Scale's formula.) 



Carmine, 5 grains. 



Glycerine, with 8 to 10 minims of acetic acid, ^ ounce. 



Glycerine, 1 ounce. 



Alcohol, 2 drachms. 



Distilled water, 6 drachms. 



Ammonia, a few drops. 



Mix the carmine with a few drops of water, and 

 when thoroughly incorporated, add 5 minims of 

 liquor ammonia. Add about half an ounce of the 

 glycerine, and shake the mixture well in a bottle. 

 Next, pour in very gradually the acid glycerine, 

 frequently shaking the bottle during admixture. 

 Test the fluid from time to time with blue litmus 

 paper, and if not of a very decidedly acid reaction, 

 add a few drops more acid to the remainder of the 

 glycerine and mix as before. Lastly, add the 

 alcohol and water, very gradually, shaking the 

 bottle thoroughly after the addition of each succes- 

 sive portion, until the whole is well mixed. 



PRUSSIAN BLUE FLUID. (Dr. Beale's formula.) 



Wood-naphtha, or pyro-acetic spirit, 1| drachm. 



Glycerine, 2 ounces. 



Alcohol, 1 ounce. 



Ferrocyanide of Potassium, 12 grains. 



Tincture of Perchloride of Iron, 1 drachm. 



Distilled Water, 3 ounces. 



Dissolve the ferrocyanide of potassium in 1 

 ounce of the glycerine, mix the tincture of iron 



