72 METHODS OF MICROSCOPICAL RESEARCH. 



water-bath. Now place the rabbit into a warm 

 water-bath of a temperature of from 40 to 45 C., 

 and let it remain for from 10 to 15 minutes, i.e., 

 until thorougly warmed. The entire abdomen may 

 be carefully opened along the middle line to allow 

 the warm water to surround the internal organs, 

 and to ensure the equable flow of the injected fluid 

 into all parts of the body. 



When the animal is thoroughly warm the 

 syringe, which should also have lain for some time 

 in warm water is to be carefully filled with the 

 injecting fluid, in which its nozzle is to be entirely 

 immersed, whilst the piston is worked several times 

 to ensure the expulsion of all air. It is then placed 

 in the stopcock, which should, however, first be 

 filled with fluid from the syringe to prevent the 

 introduction of any air, and which has already been 

 inserted into the neck of the cannula to receive the 

 nozzle of the syringe, and which, stopcock, is to be 

 turned off each time it may be necessary to refill 

 the syringe ; now with a gentle equable pressure 

 the fluid is to be injected into the animal until the 

 entire system of blood vessels shall be filled, but not 

 distended. The operator from time to time examines 

 the head, opens the eyes and parts the lips of the 

 animal to watch the progress of the work. If the 

 fluid is flowing properly, the mouth will almost 

 immediately exhibit signs of the colour with which 

 the injection is being made, and which will first be 

 seen in the gums of the lower incisor teeth. When 

 the eyeballs show the finest lines of colour traversing 

 them, the capillaries may be considered to be filled 

 and the injection complete and perfect. 



The animal is now to be put into cold water in a 



